Microbiology

ISOLATION OF PURE CULTURES A pure floriculture theoretically contains a single bacteriuml species. There are a number of procedures available for the isolation of pure cultures from mixed populations. A pure culture may be keep apartd by the use of special media with specific chemical or swagger agents that allow the enrichment or selection of one organism oer another. The differential and selective procedures will be utilized after in this course. Simpler methods for isolation of a pure culture fit out: (i) spread plating on solid agar-agar culture medium with a drinking glass broadcaster and (ii) bar plating with a loop. The purpose of spread plating and stripes plating is to isolate individual bacterial cells (colony-forming units) on a solid food medium. two procedures (spread plating and streak plating) entreat understanding of the aseptic technique. antisepsis can be specify as the absence of morbific microorganisms. However, the term is usually utilise to any technique knowing to keep unwanted microorganisms from dirty unproductive materials. FIRST consequence Material: 1. 2. 3. 4. 5. Seven 9-ml dilution tubes of sterile saline Seven nutrient agar plates 1.0 ml and 0.1 ml pipets Glass spreader aka field hockey stick 95% ethyl alcoholic drink in glass beaker (WARNING: halt alcohol away from flame!!) 6.

Mixed nightlong parentage culture of Staphylococcus aureus and Serratia marcescens 27 Procedure: (work in pairs) A. Spread Plate Technique In this technique, the number of bacteria per unit volume of sample is reduced by subsequent dilution before the sample is sp read on the surface of an agar plate. 1. Pre! pare serial dilutions of the broth culture as shown below. Be sure to mix the nutrient broth tubes before to one by one one serial transfer. Transfer 0.1 ml of the final triad dilutions (10-5, 10-6, 10-7) to each of three nutrient agar plates, and label the plates. 2. bewilder the beaker of alcohol containing the glass spreader away from the flame. Remove the spreader and genuinely carefully pass it over the flame...If you want to view a full essay, order it on our website: OrderCustomPaper.com

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